In addition to our groundbreaking research, the Camps laboratory has published methods articles detailing experimental protocols. These articles encompass various techniques, such as error-prone Pol I mutagenesis in vivo, monitoring mutagenesis in liquid culture, and performing conjugation assays. Furthermore, we are actively investigating how experimental conditions impact the efficiency of conjugation, providing valuable insights for future studies in the field.
Detection of conjugative plasmids and measurement of conjugation efficiency
Mota-Bravo et al. 2023. J Vis Exp. (193). doi: 10.3791/64523. PMID: 37036197.
Experimental setting used to detect conjugative plasmids
Quantitative measurement of mutagenesis in E. coli
Standley et al. 2017. Methods Enzymol. 591:159-186. doi: 10.1016/bs.mie.2017.03.013. PMID: 28645368; PMCID: PMC5747304.
Method for detection of two mutation separated in time
Random mutant library generation by error-prone Pol I replication in E. coli
Alexander et al. 2014. Methods Mol Biol.1179:31-44. doi: 10.1007/978-1-4939-1053-3_3. PMID: 25055769; PMCID: PMC4112372.
Diversity in individual colony fluorescence intensity
Random mutant library generation by error-prone Pol I replication and measurement of drug resistance using drug gradients
Troll C et al. 2011. J Vis Exp. (49):2505. doi: 10.3791/2505. PMID: 21445044; PMCID: PMC3376869.
Comparison between liquid and direct plating mutagenesis protocols
